专利号:US-7344863-B2 优先权日:1998-03-13 标题:Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules 发明人:LI WU-BO; JESSEE JOEL A; SCHUSTER DAVID; XIA JIULIN; GEBEYEHU GULILAT 权利人:INVITROGEN CORP 摘要:The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.
专利号:US-10865116-B2 优先权日:2017-03-24 标 题:Process for the synthesis of IZM-2 zeolite in the presence of a template, 1,6-bis(methylpiperidinium)hexane dihydroxide 发明人:MARTINEZ FRANCO RAQUEL; BATS NICOLAS 权利人:IFP ENERGIES NOW 摘要:A novel process is described for the preparation of a microporous crystalline solid, known as IZM-2 microporous solid or IZM-2 zeolite. This novel process consists of carrying out the synthesis of IZM-2 zeolite by conversion/transformation of a zeolite with structure type FAU in a fluorinated medium under hydrothermal conditions. In particular, said novel process consists of carrying out the synthesis of an IZM-2 zeolite in a fluorinated medium starting from a zeolite with structure type FAU used as the source of silicon and aluminium and a specific organic molecule or template comprising two quaternary ammonium functions, namely 1,6-bis(methylpiperidinium)hexane dihydroxide.
专利号:US-10875778-B2 优先权日:2017-03-24 标 题 :Process for the synthesis of IZM-2 zeolite in the presence of a template, 1,6-bis(methylpiperidinium)hexane dibromide 发明人:MARTINEZ FRANCO RAQUEL; BATS NICOLAS 权利人:IFP ENERGIES NOW 摘要:A novel process is described for the preparation of a microporous crystalline solid, known as IZM-2 microporous solid or IZM-2 zeolite. This novel process consists of carrying out the synthesis of IZM-2 zeolite by conversion/transformation of a zeolite with structure type FAU under hydrothermal conditions. In particular, said novel process consists of carrying out the synthesis of an IZM-2 zeolite starting from a zeolite with structure type FAU used as the source of silicon and aluminium and a specific organic molecule or template comprising two quaternary ammonium functions, namely 1,6-bis(methylpiperidinium)hexane dibromide.
专利号:US-5684191-A 优先权日:1996-10-31 标 题 :Process for the combined synthesis of betaine and choline chloride 发明人:BELLIS HAROLD EDWARD; JEMISON THOMAS ROBERT; MATHRE OWEN B 权利人:DUCOA LP 摘要:A process for the combined synthesis of betaine and choline chloride from monochloroacetic acid, trimethylamine and ethylene oxide by first reacting trimethylamine with monochloroacetic acid, and then reacting ethylene oxide with the reaction product from the previous step.
专利号:US-4997991-A 优先权日:1990-03-26 标 题 :Synthesis of diethynylbenzene 发明人:CAROSINO LAWRENCE E; HERAK DAVID C 权利人:HERCULES INC 摘要:The invention provides an inexpensive synthesis process for preparation of diethynylbenzene monomers that are useful in the preparation of polyacetylenes. This process provides for the preparation of thermally sensitive monomers in a one-pot reaction using readily available materials at low temperatures in an environment capable of absorbing large amounts of energy. Divinylbenzene is first brominated and then dehydrobrominated with sodium hydroxide or potassium hydroxide preferably in the presence of a phase transfer agent followed by distillation to recover the diethylnylbenzene product.
专利号:US-5529929-A 优先权日:1995-06-07 标 题 :Optical resolution of alkyl 1,4-benzodioxan-2-carboxylates using esterase from serratia marcescens 发明人:ROSSI JR RICHARD F; ZEPP CHARLES M; HEEFNER DONALD L 权利人:SEPRACHEM INC 摘要:A process for resolving racemic alkyl 1,4-benzodioxan-2-carboxylates useful as intermediates in the synthesis of optically pure pharmaceutical compounds such as (S)-doxazosin is disclosed. The process utilizes a microbial enzyme derived from Serratia marcescens to catalyze the enantioselective hydrolysis of the alkyl (S)-1,4-benzodioxan-2-carboxylate enantiomer of the racemic mixture to its corresponding carboxylic acid at a faster rate than the R-enantiomer. An enantiomerically pure S-configured carboxylic acid is thereby formed for subsequent pharmaceutical synthesis. The nonhydrolyzed alkyl (R)-1,4-benzodioxan-2-carboxylate enantiomer can also be isolated and racemized, and the enzymatic hydrolysis reaction repeated.